Coding

Part:BBa_K2201221:Design

Designed by: Yannic Kerkhoff   Group: iGEM17_Bielefeld-CeBiTec   (2017-09-23)


CDS of GFP-streptavidin fusion protein with medium gly-gly-ser linker containing an amber-codon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 787
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 829
    Illegal AgeI site found at 880
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

Remove stop-codon at GFP and start-codon at streptavidin.


Source

GFP extracted from E0040 and streptavidin extracted from J36848.

References